UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Allied Biochemistry-I
(90 hrs)

Unit - I:

Definition and classificationof carbohydrates, linear and ring forms (Haworth' s formula) for all monosaccharides(glucose,fructose,mannose ) and disaccharides (maltose, lactose,sucrose). Physical properties-mutarotation,chemical properities, ten reactions of glucose and four reactions of fructose (oxidati on,reduction,osazone formation, Seliwanoff's reaction), Disaccharide-maltose, lactose, sucrose-structure, occurrence, physical and chemical properties. (18 hrs)

Unit - II

Polysaccharides-starch, glycogen. cellulose, structure and properities.Glycolysis, TCA cycle, energy yield, HMP pathway. (18 hrs)

Unit - III

Amino acids,various classifications,non-protein amino acids-structure,essential and non essential amino acids,amphoteric nature, i soelectric point. Reactions due to carb oxyl, amino and both the groups. Proteins-classification-shape, solubility and composition, biological functions. (18 hrs)

Unit - IV

Proteins,physical properties-ampholytes,isoionic point,salting in and salting out, denaturation, peptide bond. Structure-primary, end group analysis, N-terminal- Edman's method,dansyl chloride method, c-terminal-hydrazinolysis and biochemical method. Amino acid sequence determination, secondary structure- helix and Pleated sheet, tertiary structure,various forces involved-quarternary structure. Deamination,transamination and urea cycle. (18 hrs)

Unit - V

Fat - function, classification, simple lipids, fatty acids (saturated and unsaturated) compound lipids, derived lipids, properties-saponification, rancidity, reduction, oxidation, halogenation. Functions of Phospholipids. Cholesterol structure - biological importance, chemical properties. Bile salts-function. (18 hrs)

Books recommended:

1. Fundamentals of Biochemistry- J.L.Jain.
2. Text book of Biochemistry-A. V. S .Rama Rao
3. Fundamentals of Biochemistry-A. C.Deb