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Semester V - Paper VIII - Molecular Biology II
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University of Madras - Syllabus of Bachelor of Science (BSc) Bio-Chemistry - Semester V - Paper VIII - Molecular Biology II

UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Semester V - Paper VIII - Molecular Biology II

Duration: 3 hrs
Maximum Marks: 100
Credits: 4

(60 hrs)

Unit I

Transcription-Prokaryotic RNA polymerase-Enzyme structure, role of sigma factor, promotor, closed and open promoter complexes.

Initiation, elongation and termination of RNA synthesis. (12 hrs)

Unit II

Eukaryotic RNA polymerases- I,II,III.

Post transcriptional modification in Prokaryotes. Post transcriptional modifications of mRNA,rRNA and tRNA. Inhibitors of transcription. (12 hrs)

Unit III

Genetic Code-Genetic Code: Basic features of genetic code. Deciphering of Genetic code. Wobble Hypothesis. (12 hrs)

Unit IV

Protein biosynthesis-activation of amino acids, initiation, elongation and termination of Translation in prokaryotes.

Post translation modifications. Inhibitors of translation. (12 hrs)

Unit V

Regulation of gene expression in prokaryotes Operon- Concept- Positive and negative regulation of lac operon. (12 hrs)

BOOKS RECOMMENDED

1. Biochemistry of nucleic acids- Adam et al
2. Molecular Biology - David Friefelder
3. Harper's Biochemistry - Harper et al
4. Molecular Biology of the gene - James and Watson
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