Syllabus of Jawaharlal Nehru University MSc Biotechnology - BT515 - Enzymology

Credits: 2

1. Enzymes

General characteristics and Catalytic power of enzymes and their classification
Energy considerations
Factors affecting enzyme activity
Enzyme kinetics, Michaelis-Menten equation
Allosteric enzymes and their regulation
Enzyme inhibition, activation of enzymes
Immobilized enzymes
Different mechanisms of enzyme catalysis acidbase and covalent catalysis
Molecular mechanism of action of chymotrypsin
Lysozyme and carboxy peptidase
Structure-function relationship of enzymes

2. Biological membranes : Structure, function and dynamics

3. Transport of biomolecules into the cells

4. Signal Transduction cascades

Receptor higgerd phosphorylation cascade, G protein cascade, cyclic CAMP as a second messenger, protein kinases, receptor higgard hydrolysis of phosphotidyl inositol, IP3, Diacyl glycerol, calcium ion as a cytosolic messenger, Calmodulin

5. Secondary metabolites with special reference to antibiotics and microbial toxins