Syllabus of Jawaharlal Nehru University MSc Biotechnology - BT518 - Laboratory Techniques-I

Credits: 4

General

a. Making of buffers

b. Titration of Polybasic acids and evaluation of pKa's

c. Titration of amino acids

d. Two dimensional TLC of amino acids

e. Paper chromatography of carbohydrates

Proteins :

a. Isolation of proteins

b. Estimation of proteins by Lowry and Brandford methods

c. Gel filtration, ionexchange and affinity chromatography

d. Gel Electrophoresis

e. Wavelength scan of proteins

f. Thermal unfolding of proteins and calculations of thermo-dynamic parameters from temperature scanning UV spectrophotometer, Effect of solvent conditions on thermal stability of proteins.

g. pH titrations of protein, calculation of net charge and total charge at a particular pH.

h. Reduction of disulphide bonds of proteins.

DNA and RNA

a. Isolation of DNA and RNA

b. Estimation of DNA and RNA by chemical means

c. Wavelength scan of DNA and RNA

d. Melting studies of calf thymus DNA

Carbohydrates:

a. Estimation of glucose by Glucose oxidase (Trinder's reagent)

b. Estimation of reducing sugars by Nelson Somogyi's method

c. Effect of temperature, time and substrate concentration on salivary alpha amylase activity.