Syllabus of Jawaharlal Nehru University MSc Biotechnology - BT522 - Bioprocess Technology

Credits: 3

Growth Rate Analysis

Growth rate parameters : Specific growth rate, doubling time, validity of exponential growth law, growth yield, metabolic quotient, Effect of substrate concentration, Monod Kinetics, Determination of K_s, Definition of lag period.

B. Estimation of Biomass

Factors influencing choice of method; Dry and wet mass, volume, yields, metabolic rates light scattering cell count.

C. Stoichiometry

Balance equation, carbon, Nigrogen balance, oxidation - reduction principles, product formation.

D. Batch & Plug Flow Culture

Open and closed systems, growth phases, Mathematical model of simple batch culture, variations in actual practice plug flow culture with and without feedback.

E. Chemostat Culture

General principle, Balance equations critical dilution rate, Biomass productivity, comparison with batch cultures, residence time distribution, Test of validity, imperfect mixing, wall growth Transient state analysis.

F. Elaboration of Chemostal

Turbidostat, pH stat, D.O. stat cultures control mechanisms, Biomass feedback Internal and External, Chemostat in series Applications.

G. Computer Simulation

Programming with ISIM, writing model equations, Material balance concepts, Kinetic expressions.

H. Energy Requirements

Electron transfer concept; maintenance energy, magnitude and control of maintenance energy, Effect of maintenance.

I. Product Formation in Microbial Cultures

Growth associated and non-growth associated Kinetics, Product decomposition, Balance equations for batch and continuous systems, Effect of environmental conditions.

J. Effect of Inhibition and Activation of Growth

Competitive and non-competitive inhibition, Product and substrate inhibition, activators, Effect on batch and continuous systems.

K. Fed-Batch Culture

Analysis, repeated fed-batch culture applications, dialysis culture.

L. Mixed Cultures

Different models and their analysis.

M. Oxygen Demand and Supply

Oxygen demand, solubility, measurement of D.O.T. Redox potential oxygen transfer, measurement of K_La.

N. Aeration and Agitation

Agitation and mixing, Baffled, vortex and airlift systems, Impeller design, Effect of stirring, sparging and other parameters.

O. Scale up Principles

Methods of scale up and their analysis.

P. Process Control

Measurement and control of bioprocess parameters like temp., D.O., speed, pH, antifoam etc., Basic principles of feedback control, Proportional, Integral and derivative control.