Syllabus of Jawaharlal Nehru University MSc Biotechnology - BT532 - Molecular Biology of Eukaryotic Systems

Credits: 2

1. The Cell and the Eukaryotic Genome

2. The chromosomal DNA and proteins, Repetitive and non-repetitive sequence, Exons, Introns.

3. Chromatin Structure :The decondensed and active chromatin. The nucleosome

4. The Nuclear Matrix

5. Eukaryotic promoters and enhancers.

6. Eukaryotic RNA Polymerases I, II and III, The CTD domain

7. Transcription complexes, general Transcription Factors minimum Basal Unit.
Fractionation and assembly of Basal Factors.

8. TBP's (TATA-binding Proteins) structure/function.

9. TAF's (TBP-Associated Proteins) structure and function.

10. Elongation and Termination.

11. Transcriptional Activators and repressors

12. Transcriptioanl activation and transcriptional repression

13. Genome wide location analysis: serial regulation of transcriptional regulators

14. Structure and function of activators and repressors.
Symmetry in Cis-element, Helix turn Helix.

15. Zinc fingers and Leucine Zippers.

16. Chimeric activators, Squelching.

17. Protein-Protein Interaction : Role of dimerization.
Heterologous gene Activation : Trans-& cis-inhibition.

18. DNA Structure and Gene regulation

19. Molecular chaperones and gene Expression

20. Gene expression vs gene silencing

21. RNA Processing : Processing of Precursor RNA.
RNA splicing, Splicesomes, Lariat Formation.
RNA Editing.

22. Programmed cell death (PCD)
Genetic pathways for PCD
Anti-and-pro-apoptotic proteins

23. Apoptotic signaling in response to DNA damage

24. Oncogenes and tumor suppressor genes
Tumor suppressor gene and signal transduction