Syllabus of Jawaharlal Nehru University MSc Biotechnology - BT533 - Genetic Engineering & Applications

Credits: 3

1. DNA: The Structure and Properties

2. The restriction Enzymes

3. The plasmids and Cosmids, Design of Cloning and expression vectors

4. The isolation of plasmid and genomic DNA

5. The construction of cDNA and genomic libraries

6. The labeling of DNA with radiolucleotides

7. The screening of libraries: Oligonucleotide, cDNA and antibody probes

8. The Southern, Northern, Western, South western, and Far western blotting.

9. Analysis of DNA-Protein Interactions

10. Electromobility shift assay, Methyl Interference assay, DNase Footprinting

11. Protein-protein interactive cloning: The yeast two hybrid system

12. PCR and its applications

13. Fidelity of DNA polymerases

14. The use of PCR in gene assembly: multiplex, nested, RT PCR, overlap extension and SOEing

15. PCR in gene recombination: Deletion, recombination, addition, and Site-specific mutagenesis

16. PCR in molecular diagnostics

17/18. Detection of hepatitis, herpes, HIV, and EBV

19.The role of PCR in detecting minimum residual diseases (MRD)

20. Gene Disorder

21. Detection of mutation in neoplastic diseases

22. SSCP, RFLP, ASA, DGGE

23. MCC, RNAseA, OLA, PTT

24. Differential gene expression profiling by Microarray

25. Differential protein expression profiling

26. Recombinant protein technology : Design and use of expression vectors.

27. Expression of foreign gene in E. coli, Baculovirus and Pichia expression system.

28. Inclusion Bodies and regeneration of active proteins.

29. Method for cell synchronization.

30. Methods of introduction of DNA into mammalian cells.

31. Transient and stable integration of foreign DNA into mammalian cells, Single cell cloning

32. The design of viral vectors : The replication competent and the replication deficient retrovirus.

33. The decision of Adeno viral, unarmed Herpes and vaccinia viral vectors.
The packaging of retroviral vectors.

34. Gene knockouts in animals.

35. Gene therapy:Somatic and germ line gene therapy in vivo and ex-vivo

36. Design of SiRNA vectors and Gene silencing