UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BOTANY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Semester VI - Paper XII - Plant Biotechnology

Duration: 3 hrs
Maximum Marks: 100
Credits: 4

Lecture 4 Hrs; Practicals 2 Hrs. per week

Unit - I

Biotechnology - definition, demand for biological resources, achievements. Genetic engineering - aims of genetic engineering, techniques of gene manipulation. Recombinant technology, outline of cloning - plasmid, cosmid, vectors.

Unit - II

Fermenation technology, microbial growth, batch culture, continuous culture, fermentor designs and operators. bioreactors, advantages of microbial enzymes, industrial applications.

Unit - III

Plant cell and tissue culture - totipotency, nutrient medium - MS & B5 media, sterilization, inoculation and maintenance of culture. Micropropagation - root and shoot culture and its uses. protoplast culture, isolation, purification and regeneration.

Unit - IV

Protoplast fusion - somatic hybrid, somoclonal embryos. Synthetic seeds. Application of PTC (Plant Tissue Culture) in agriculture, horticulture and forestry.

Unit - V

SCP - micro organisms - nutritional value - production of algal biornass. Bio fertilizers and bio pesticides - mass cultivation of Rhizobium, Azatobacter, Azospirillum, Mycorrhiza, Bluegreen algae and Azolla.

Practicals

1. Preparation of different nutritive media of PTC - preparation of explant callus culture.
2. Observe and note the importance of Agar, Azolla, Spirulina, Yeast, Biofertilizers.
3. Cultivation of Azolla

Demonstration

Synthetic seeds, immobilization technique using alginate (blue green algae).

Reference Books

1. Dubey R.C 1993, A Text Book of Biotechnology, S.Chand & Co Ltd, New Delhi.
2. Treven, M.D.S Buffeiy, R.G. Goulding and F Standury 1987, Biotechnology, The Biological Principles, Tata McGraw Hill, New Delhi.
3. Brown C.W, I.Cambell and F.G Priest 1987, Introduction to Biotechnology, l3lackwekk Scientific Publishers, Oxford.
4. lgiacimuthu S 1996, Basic Biotechnology, Tata McGraw Hill Publishing Co Ltd New Delhi.
5. Butcher D.N and D.S Ingram 1992, Plant Tissue Culture, Oxford.
6. Dixon R.A 1995, Plant Cell Culture, A Practical Approach, IRL Press, Oxford
7. Kumar H.D 1993, A Text Book of Biotechnology, East West Affiliated Press Ltd. New Delhi.
8. Kumar U Synthetic Seeds for Commercial Crop Production, Studies in Biochemistry Series No.1, Agrobotanica, Bikaner.

Reference

1. Dodds J.H and L.W Roberts 1985, Experiments in Plant Tissue Culture, Cambridge University Press, London.
2. Michel A, Borowitzka and Lesley, J.Borowitzka, Microalgal Biotechnology.
3. BalI A.T, Hol.G. Lilly M.D 1983, Biotechnology, International Trends and Prospectives, Oxford and IBH, New Delhi.
4. Swaminathan M.S 1992, Biotechnology in Agriculture, Macmillan India Ltd, Madras.
5. Narayanaswamy S, 1994, Plant Cell and Tissue Culture, Tata McGraw Hill Publishing Co Ltd, New Delhi.
6. Swammathan MS 1995, Farmer's Rights and Plant Genetic Resources, Reconginition and Reward, Macmillan india Ltd, Madras.

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