University of Madras - Syllabus of Bachelor of Science (BSc) Microbiology - Allied Subject - MAC-II - Bio-Instrumentation
UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN MICROBIOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS
Allied Subject - MAC-II - Bio-Instrumentation
Principles of Bio instrumentation and Techniques
Unit I :
Colorimetry and spectrophotometry.
Spectrofluorimetry, turbidometry, nephelometry, luminometry.
Electrophoretic techniques : proteins and nuclei acids
Chromatography: adsorption, partition, gas, ion, exchange, gel filtration, affinity, HPLC, FPLC
Radioistope techniques : nature of radioactivity, detection, measurements, counters, safety
aspects
Biosensors. Centrifugation and ultra centrifugation.
Microscopy light, phase contract, fluorescence, dark field, electron microscopy
Laser confocal microscopy and digital image analysis
Animal tissue culture
Decontamination, sterilisation and disinfection
Chemical synthesis of nucleotides and peptides
Sequencing of proteins and necleic acids
Cytogenetics.
Enzyme purification and assay techniques.