UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN MICROBIOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Application Oriented Subjects - MAO-IV - Fermentation technology and Downstream Procession

Unit I :

General Considerations : Metabolic pathways and metabolic control mechanisms, Primary and secondary metabolites.

Unit II :

Fermentation in batch culture : Microbial growth kinetics, measurement of growth (Cell number, direct and indirect methods) growth and nutrient, growth and product formation, heat evolution, effect of environment (temperature, pH, high nutrient concentration) Media formulation. Sterilization, kinetics of thermal death of micro-organisms, batch and continuous sterilization.

Unit III :

Continuous culture: Continuous culture system, productivity, product formation.

Unit IV :

Aeration and agitation, power requirement oxygen transfer kinetics, concepts of Newtonian and Non-Newtonian fluids, plastic fluids apparent viscosity, oam and antifoam.

Unit V :

Scale - up, instrumentation control, Physical and chemical environment sensors, downstream process.

Recommended Books and Literature Retrieval Facilities:

Text books:

Prescott, L.M.; J.P. Harley and D.A.Klein. 1993. Microbiology. 2^nd edition. Wm.c. Brown publishers.

Ketchum, P.A. 1984. Microbiology: Concepts and Applications. John wiley and sons,. Newyork.

Brock, TD. Smith D.W. and madigan M.T. 1984. Biology of Micro organisms. 4^th edition. Englewood, cliffs. N.J. Prentice Hall.

Salle, A.J. Fundamental principles of Bacteriology. 7^th edition. Tata McGraw Hill Publishing Company Ltd.

Joel Mandeistam; Kenneth McQuillen and Ian Dawes. 1992. Biochemistry of Bacterial growth. 3^rd Ed. Blackwell Scientific Publications.

Doelle, H.W. 1975. Bacterial Metablism. 2^nd Ed. Academic Press.

Moat, A.G. arid J.W. Foster. 1988. Microbial Physiology. 2^nd Ed. John Wiley and Sons.

Roitt, I.M. 1988. Essential Inununology. Biackwell Scientific Publishers.

Jewetz, E: J.L. Melnic and E.A. Adelberg (2000). Review of Medical Microbiology. 19^th edition. Lange medical Publications. U.S.A.

Ananthanarayan R. and C.K. Jeyararn Paniker, 1994. Text book of Microbiology. Orient Longman.

Jeyaram Paniker, C.K. Text book of Parasitology. Jaypee brothers. New Delhi.

Alexender, M. 1977. Introduction to soil Microbilolgy. NY. John wiley and sons.

Stanbuiy, P.F. A. Whitaker and S.J.Hall. 1995.

Principles of Fermentation Technology. 2 Ed. Pergamon press.

Frazier, W.C. and Westhoff, D.C. 1988. Food Microbiology. 4^th Ed. McGraw Hill, NY.

Old, R and Primrose, S.B. 1995. Principles of Gene manipulation. An Introduction to Genetic Engineering. 5^th Ed. Blackwell Scientific Publications, London.

Freifelder. D. 1995, Molecular Biology. Narosa Publishing House, New Delhi.

Timbury M.C. 1986. Medical Virology, 9^th Edn. Churchil Livingston London.

Diagnostic Microbiology - Fine gold 10 th Edn. CV. Mosby Company, St. Louis. (2000)

Jagadish Chandar, 1996. A Text book of Medical Mycology. Interprint. New Delhi.

Microbiology Laboratory Manual. T.Sundararaj. Published by A. Sundararaj. Chennai - 600 096.

Medical Microbiology. David Greenwood, Richard B Slack and John F. Peutherer. Churchill Livingstone (London) 16^th Edition, 2002.

Text Book of Microbiology.

D.R.Arora. CBS Publishers & Distributors, New Delhi (2^nd Edition) 2003.

Medical parasitology. D.R. Arora & B. Arora. CBS Publishers & Distributors, New Delhi (1^st Edition) 2002.

Microbiology Laboratory Munual. T.Sundararaj. Ms. Aswathy Sundararaj, Chennai (st Edition) 2002.

Practical Microbiology. R.C. Dubey and D.K. Maheshwari. S.Chand & Co. Ltd, NewDeihi (1^st Edition) 2002.

A text book of Microbiology. R.C. Dubey and D.K.Maheswari. S.Chand & Co. Ltd., New Delhi (1^st Edition) 2003.

Essentials of Biochemistry. U.Sathyanarayana. Books and Allied (P) Ltd., Kolkata (1^st Edition), 2002.

Concepts of Biochemistry. A.C. Deb. Books and Allied (P) Ltd.. Kolkata (1^st Edition), 1999.

Practical Medical Microbiology. Mackie & Mccartney. J.G.College / A.G. Fraser, B.P. Narmion / A.Simmons (14^th Edition), 1996).

REFERENCE BOOKS:

Rose, A.H. 1976. Chemical Microbiology: An Introduction to Microbial Physiology. 3^rd edition, New York. Plenum.

Gottschalk, G. 1986. Bacterial Metabolism. 2^nd Ed. Springer Verlag. NY.

Krieg N.R. and J.G. Holt. 1986. Ed. Bergeys Manual of Systematic Bacteriology.

Davis B.D.R.Delbecco, H.N. Eisen and H.S. Ginsburg. Microbiology. 1980. 3^rd edition, Newyork: Harper and Row.

Alexopolus C.J. and Mims C.W. 1979. Introductory Mycology. 3^rd Edition. Wiley, NY.

Schmidt G.D. and Roberts L.S. 1981 - Foundations of Parasitology. 2^nd Edition, Mosby, St. Louis.

Tizard, R.I. 1983. Immunology: An Introduction. Saunders college Publishing, Philadelphia. Kuby, J. 1994. Immunology. 2^nd edition. H.W. Freeman and company, New York.

Klaus D. Elgert. 1996. Immunology. Understanding the Immune System. Wiley -Liss. NY.

Lynch, J.M. and Poole, N.J. 1979. Microbial Ecology. A. Conceptual Approach. Blackwell Scientific Publications, London.

Rheinheimer, G. 1980. Aquatic Microbiology, 2^nd Ed. John Wiley & Sons, Chichester & London.

Atlas, R.M. and Bartha R. 1992. Microbial Ecology: Fundamentals and Applications. 2^nd Ed. Redwood city. CA. Benjamin / Cummings.

Mitchell, R. 1974. Introduction to Environmental Microbiology. Prentice - Hall. Inc. Englewood Cliffs - New Jersey.

Subba Rao, N.S. 1995. Soil Microorganisms and Plant Growth Oxford & IBH Publishing Co. Pvt. Ltd.

Paul, E.A. and Clark, F.E. 1989. Soil Microbiology and Biochemistry, New York, Academic Press.

Sabha Rao, N.S. 1995. Biofertilizers in Agriculture and Forestry. 3^rd Ed. Oxford & IBH Pub. Co. Pvt. Ltd. New Dethi.

Banwart, G.J. 1989. Basic Food Microbiology. CBS Publishers and Distributors. Casida, J.E. 1968. Industrial Microbiology. Wiley Eastern.

Adams, M.R. and M.O. Moss. 1995. Microbiology. New Age International (P) Limited Publishers.

Winnackr, E.L. 1987. From Genes to clones. Introduction to Gene Technology. VCH, Weinheim.

Glover, D.M. 1984. Gene Cloning. The Mechanism of DNA Manipulation. Chapman and Hall. Brown, T.A. 1995. Gene Cloning. Chapman and Hall

Maloy, S.R: Cronan, J.E.Jr. and David Freifelder. 1994. Microbial Genetics. 2^nd Ed. Jones and Bartlett Publishers.

Walter Beck J and J.E. Davies 1976 Medical Parasitology. 2^nd Ed. C.V. Mosby Company St. Louis.

Robert Desowitz 1980 Ova and Parasites, Harper and Row Publishers, New York.

R.Levanthal, R.S. Cheadle 1979. Medical Parasitology S.A. Davies Co., Philadelphia.

Chatterjee. Medical Parasitology

Jayaram Panikar. Text book of Parasitology

Grierson, D and S. Covey, 1989. Plant Molecular Biology. 2^nd Ed. Blackie, London.

Freifelder, D. 1995. Microbial Genetics. Narosa Publishing House, New Delhi.

Luria, S.E. Darnel, J.E. Jr. Baltimore, D and Campbell, A. 1978. General Virology. 3^rd Ed. New York. John Wiley & Sons.

Fenner et al. The Biology of Animal Viruses. Academic Press. New York.

Hayes, W. 1968. The Genetics of Bacteria and their viruses. Blackwell Publications, London.

Edward A. Bridge. 1994. Bacterial and Bacteriophage. Genetics 3^rd Ed. Springer-Verlag. Lennette, E.H. 1974. Diagnostic Procedures for Viral and Rikettsial Diseases. American Public Health Association, NY.

Hoeprich PD 1977 Infectious Diseases 2^nd Edn. Harper & Row Publishers.

Glick B.K. and Pasternak, J.J. (1999) Molecular Biotechnology. Principles and Applications of Recombinant DNA. ASM Press, Washington, DC.

Winnacker, E.L. 1987. From Genes to Clones. Introduction to Gene Technology. VCH Weinheim.

Kwon - Chung KJ and JE Bennet Medical Mycology, Lea & Febiger, London.

Microbiology 4^th Edn. Davis, Dulbacco, Harper International Edition. Infectious Diseases - Hoeprich, Harper Row Publishers.

Stryer (1995). Biochemistry, W.H. Freeman & co. 1064 pp.

Introduction to Microbiology

John L. Ingraham & Catherine A. Ingrahani.

Books / cole Thomson Learning, UK (2^nd edition) 2000