UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN MICROBIOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester II - Practical - MCC-203 - Basic Techniques in Microbiology and Microbial Physiology

Duration of Examination: 3 hrs
Max Marks: 100
Credits: 4

1. Cleaning of glasswares.

2. Sterilisation principle and methods - moist heat - dry heat and filtration methods.

3. Media preparation : Liquid media, solid media, Agar slants, Agar plates. Basal, enriched, selective media preparation - quality control of media, growth supporting properties, sterility check of media.

4. Pure culture techniques: Streak plate, pour plate, decimal dilution.

5. Cultural characteristics of microganisms : Growth on different media, growth characteristics and description. Demonstration of pigment production.

6. Microscopy: Light microscopy, bright field microscopy, dark field microscopy.

7. Motility demonstration : Hanging drop preparation, wet mount, dark field microcopy, semisolid agar, Craigies tube method.

8. Staining techniques : Smear preparation, simple staining, Gram’s staining, Acid fast staining, Staining for metachromatic granules.

9. Morphology of microorganisms : Morphological variations in alage. Morphology of fungi, slide culture techniques.

10. Antibiotic sensitivity testing: Disc diffusion test - Quality control with standard strains.

11. Micrometry: Determination of size of yeast. Fungal. filaments.

12. Physiology characteristics : IMViC test, H₂S, Oxidase, catalase, urease test, Carbohydrate fermentation, Maintenance of pure culture, Paraffin method, Stab culture, maintenance of mold culture.

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