UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN MICROBIOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester IV- Practical - MCC-403 - Basics and Applied Immunology

Duration of Examination: 3 hrs
Max Marks: 100
Credits: 4

1. Blood groups and Rh.typing - coomb's test.

2. Precipitation reaction in gel. Outchelony - double diffusion, single radial immuno diffusion.

3. VDRL, RPR

4. Agglutination reactions: Slide and Tube methods RBC agglutination IHA, TPHA Bacterial.

5. Complement fixation test. Titration of amboceptor complement(demonstration only)

6. Immunofluorescence, (Demonstration only)

7. ELISA

8. Isolation of Buffy coat, using heparin lymphocytes (T cells, B cells) enumeration of different cells types, Peripheral blood cell counts, absolute cell counts.

9. Antibody productions in rabbits against sheep RBC and its titration (Demonstration)

10. Anaphylactic reactions in guineapigs. Arthus reaction in rabbits. (Demonstration)

11. Skin tests. Both immediate and delayed hypersensitivity reactions to egg protains, bacterial, fungal antigens.