University of Madras - Syllabus of Bachelor of Science (BSc) Microbiology - Semester VI - Practical - MCC-604 - Medical Microbiology
UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN MICROBIOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS
Semester VI - Practical - MCC-604 - Medical Microbiology
Duration of Examination: 3 hrs
Max Marks: 100
Credits: 4
1.General requirements of collections, transport of clinical specimens - Direct examinations - staining of specimens - Methods of enriched, selective and enrichment culture techniques
used to isolate organisms from clinical materials.
2. Wet mount examinations of stool for parasites. Vaginal specimens for Trichomonas.
3. KOH and Lactophenol preparations for skin scrapings for fungi.
4. Simple, differential and special staining of clinical materials viz. Throat swab, pus, urine, sputum, stool etc.
5. Enumeration of Bacteria in urine. Quantitative urine culture.
6. Estimation of worm burden in stool. Floatation and sedimentation techniques of stool examination.
7. Germ tube, assimilation, fermentation for yeasts.
8. Isolation and identification of bacterial pathogens from clinical specimens their biochemical reactions.
9. Antimicrobial sensitivity testing by disc-diffusion tçchnique and determination of MIC.
10. Identification of pathogenic microbes including viruses in slides / smears / specimens as spotters.