University of Madras - Syllabus of Bachelor of Science (BSc) Plant Biology and Plant Biotechnology - Application Oriented Subject II - Microtechnique
UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN PLANT BIOLOGY AND PLANT BIOTECHNOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS
Application Oriented Subject II - Microtechnique
Lecture: 4 Hrs
THEORY
Unit - 1:
Light microscopy - History and - Optical principles use and care of Microscopes - A brief survey of different types of microscopes including E.M.
Unit - 2:
Microtechnical processes - principles and techniques:
(A) Fixation and Fixatives
(B) Temporary Staining and stains
Unit - 3:
Microscopic preparations, Semipermanent and Permanent
Unit - 4:
Special techniques: smear, squash & maceration
Unit - 5:
Microtomy: types of microtomes and their use.
Methods for specific materials:
(A) Whole mount of algae & fungi
(B) Sectioning of bryophytes, pteridophytes & gymnosperms
(C) Maceration & sectioning of angiosperm materials