UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN PLANT BIOLOGY AND PLANT BIOTECHNOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester V - Paper VI - Cell Biology and Molecular Biology

Lecture: 4 Hrs
Practicals: 2 Hrs per week

CELL BIOLOGY

Unit 1 :

Introduction - definition, scope, cell organization- Prokaryotic and Eukaryotic. Cell boundries, cell wall-gross layers ie middle lamella, Primary wall, secondary wall - structure, chemistry and functions of cell wall -pits (simple and bordered), Plasmodesmataplasma membrane- occurrence, structure. (light microscopic, electron microscopic and molecular) chemistry, function and origin.

Unit 2 :

Occurrence, structure, function and origin of EndOplasmic reticulum, Golgi bodies, lysosomes, Ribosomes, perioxisomes, Mitochondria and Chioroplast. DNA autonomy of organelles.

Unit 3 :

Nucleus, nuclear membrane, chrom\osomes, euchromatin, heterochromatin, giant chromosomes polytene and lampnrush. Cell cycle, cell division, mitosis and meiosis, cytokinesis.

MOLECULAR BIOLOGY

Unit 4 :

Nature and function of genetic materials-DNA- structure and replication- RNA -types and DNA repair Protein synthesis- trancription, translation, genetic code evidence of triplet nature of the code.

Unit 5 :

Gene regulation in prokaryotes-lac- operon and tryp-operon as biosynthetic system, autoregulation and feed back inhibition. Genetic engineering - recombinant DNA, isolation of DNA- vector, restriction enzymes. Tranformation- screening for recombinants.

PRACTICAL :

ITEMS INCLUDED IN THE THEORY.

BOOKS:

1. Brown W.V and Bertke. E.M. 1974, A text book of Cytology C.V Mosley Co, St.Louis
2. Cohn, N.S. 1979. Elements of Cytology. Freeman Book Co.
3. De Robritis E.D.P and DeRobritis E.M.F jr. 1987 Cell and Molecular biology Lea and Febiger.
4. Verma P.S. and Agarwal VK, 1995 Cell Biology and Evolution.
5. Freifelder.D. Essentials of Molecular Bilogy. Joner and Bartlette Pub Inc. Boston.
6 Feifelder. D. Molecular Biology. Narosa.
7. Watson J.D et.al Molecular biology of the Gene The Benjamin / Cummings.
8. Freifelder, D. Microbial Genetics Jones & Barlett Publications.