UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN PLANT BIOLOGY AND PLANT BIOTECHNOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester VI - Paper XII - Plant Biotechnology

Lecture: 4 Hrs
Practicals: 2 Hrs. per week

1. Biotechnology - definition, history and scope.

2. Isolation and cultivation of economically important microbes

a) Fresh water Alga (Scenedesmus)
b) Fungus (Aspergillus)

3. Tissue culture- media, callus formation and protoplast fusion with reference to improvement of plants.

4. Single cell protein(SCP- Micro- organism used in SCP (Scenedesmus and Spirulina). Nutritional value of SCP. Algal Biomass production and maintenance.

5. Mushroom cultivation- Paddy straw and Oyster mushrooms, Nutritional value and methods of cultivation. Control of Pests and pathogens.

6. Biofertilizers- Blue green algae, Azolla, Fungi, Mycorrhiza(VAM) Bacterium-Azosprilluum sp.

7. Production of primary metabolites- Ethanol production by Yeast, Citric acid production by Aspergillus niger.

Production of secondary metabolites- Antibiotics-Penicillin. Enzymes from microbes and their application- Amylase, Proteases, Renin, Pectinase and Glucose oxidase.

8. Improvement of plants: Alkaloid production, Vaccine production Luminescent plants, protein sweetener, conversion of photosystems C3 to C4.

8. Improvement of plants: Alkaloid production, Vaccine production Luminescent plants, protein sweetener, conversion of photosystems C3 to C4.

BOOKS:

1. Borowitzka.M.A and Borowitza.L.J. Microalgal Biotechnology.
2. Dodds. J.H and Roberts. L.W Experiments in plant tissue culture. Cambridge University Press.
3. Sasson, A. Biotechnologies challenges and promises UNESCO.
4. Srivastava. H.S. An introduction to Biotechnology.Wlly eastern.
5. Trehan. M.D. Boffey, S. Goulding.R.A. and Stanburv.P. Biotecxhnology. The Biological principles. Tata Mc Graw Hill.
6. Wiseman.A. Principles of Biotechnology. 2^nd edition Surrey University Press. UK.

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