UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN PLANT BIOLOGY AND PLANT BIOTECHNOLOGY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester VI - Practical - Plant Physiology

Practicals: 2 Hrs. per week

Experiments to be performed and recorded by students individually

1. Determination of water potential by plasmolytic method.
2. Determination of water potential by gravimetric method.
3. Effect of temperature and chemicals on membrane permeability.
4. Study of rate of transpiration under different environmental factors.
5. Determination of degree of porosity of leaves.
6. Separation of plant pigments by paper chromatography.
7. Study of rate of photosynthesis under different light intensities.
8. Study of rate of photosynthesis under different wavelengths (red &blue) of light.
9. Comparison of rate of respiration of different respiratory substrates.
10. Determination of RQ using respirometer.
11. To test the germination capacity of seeds using Tetrazolium chloride.
12. Measurement of pH of expressed cell sap and different soils using pH meter.
13. Determination of ratio of water absorption and transpiration by weighing method.

Demonstration -Experiments

1. Demonstration of stomatal movement.
2. induction of roots in leaves by auxins.
3. Amylase activity.
4. Effect of pH on catalase in leaf discs.

BOOKS:

Plant physiology:

1. Arditti, J. and Dunn, A. 1969. Experimental Plant Physiology. Holt, Rinehart and Winston.
2. Devlin, R.M. 1966. Plant Physiology. Affiliated East West Press.
3. Devlin, R.M. and Witham, F.H. 1985. Plant Physiology.
4. Noggle, G.R. and Fritz, G.J. 1982. Introductory Plant Physiology. Prentice Private Ltd.
5. Pandey, S.N. and Sinha, BK. 1985. Plant Physiology. Vani Educational Books.
6. Rao, K.N, Sudhakara Rao, g. and Bharathan, S. 1987. The functioning Plant. S. Viswanathan pvt. Ltd.
7. Salisbury, F.B. and Ross, C.W. 1986. Plant Physiology. CBS publishers and distributors.

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