UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN Zoology
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester V - Application Oriented Subject I - Medical Laboratory Techniques and Bioinstrumentation I

Duration of Examination: 3 hrs
Maximum Marks: 100
Credits: 4

Unit I

Laboratory administration, Management and safety. Safe disposal of hospital wastes. Cleaning, care and sterilization of laboratory items such as glasswares. Sterilization (flame. steam, chemical and pressure). Preparation of reagents. Preparation of culture media - vaccination and inoculation.

Unit II

Microtechniques - Tissue preparation, fixing, embedding. sectioning, staining and nountmg - vital staining.

Unit III

Clinical physiology: Composition of Blood, Clotting mechanism, Use of blood pressure apparatus, ECG and Respirometer- Blood sugar regulation - Estimation of Blood glucose - Diabetes melitus - Haemoglobin estimation - Anaemia.

Unit IV

Haeniatology - collection of blood samples, analysis of blood and basic haematological techniques. Haematocrit, packed cell volume, erythrocyte sedimentation rate, Estimation of Serum Cholesterol, Urea, Uric acid, protein and creatinine.

Unit V

Blood and cell morphology, RBC, WBC total counts and differential counts in health and disease. Platelet count, reticulocytocrit, RBC fragility test haemorrhagic disorders, clotting time, bleeding time, prothrombin time, test for deficiency in blood clotting factors.

REFERENCE:

1. Sood Ramnik, 1985. Medical Laboratory Technology, Jaypee Brothers, New Delhi, 384 p

2. King, Maurice, 1976, A Medical Laboratory for Developing Countries, Oxford University Press, ELBS Edition, Madras

3. Gadd, P., 1973. Human and Social Biology for Tropics, Macmillan Education, London, 250 pp

4. Bomford, Mason and Swash, 1975. Hutchinson's Clinical methods, Beillers Tindal, ELBS edition, 362 pp

5. Palki, Bhalchanda and Jeevaji, 1989. An Introduction to Microtechnique, S. Chand & Co., Madras, 167 pp

6. Samuel, K.M., - Notes on Clinical Laboratory Techniques, M.K.G. Iyer & Sons, Madras.

7. Swaroop, Pathak and Arora, 1981. Laboratory Techniques in Modern Biology, Kalyani Publishers, New Delhi.

8 Mathur and Garg, 1975. Laboratory Manual for General Practitioners and Health Centres, Pragati Prakashan. Meerut, 256 pp.

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