UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN Zoology
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester VI - Paper XI - Microbiology, Immunology and Biotechnology

Duration of Examination: 3 hrs
Maximum Marks: 100
Credits: 4

Unit I

History of microbiology - Biogenesis - contributions of Louis Pasteur - Leuwanhoeck, Lazaro, Spallanzani, John Tyndall, Joseph Lister, Robert Koch. Anatomy of Prokaryotes, Eukaryotes - Microbial identification - morphological, physiological, and biochemical properties. Microscopy-simple & compound light microscopy-Dark field-phase contrast- fluorescence and electron microscopy-Sterilization methods.

Unit II

Culture techniques - Media preparation-Preservation of cultures-Aerobic and anaerobic bacteria-Growth of bacteria-Microbial morphology-wet mounting drop-Staining method - dyes-simple-differential and special staining techniques-acid fast-spore-capsule stains- Microbial classification - Basic concepts of microbial metabolism - Microbiology of drinking water, waterborne diseases.

Unit III

Immunology

History of Immunology - Immunohaematology - Blood transfusion-Rh-compatibilities. Innate immunity - Acquired immunity. Structure, composition and functions of cells and organs involved in immune system - Microbial infections, virulence and host resistance related to imm

Unit IV

Antigens, types, properties, Haptens, Adjuvants - vaccines - types - Toxoids Antitoxins. Immunoglobulins, structure, types and properties. Theories of antibody production. Complement structure properties-function and pathway. Antigen-Antibody reaction-invitro methods. Agglutination-precipitation-complement fixation-immuno-fluorescenceELISA, RIA

Unit V

Biotechnology

Definition and history-scope and significance-principles and applications of genetic recombinant technology in medicine, agriculture, veterinary and food industry - Production of biotechnological Products-food SCP [algae,yeast,mushroom]-Biofertiliser-Biofuel-Biopesticides-Biogas production-waste and sewage management-Effective microorganisms-Enzyme biotechnology-sources and production of commercially important enzymes cellulase, amylase, pectinases, proteinases. Immobilization of enzymes and its applications-Genomic Library.

REFERENCE

1. Pelczar Jr.M.J.Chan E.C.S and Kreig N.R.[2001] MICROBIOLOGY-McGraw Hill Inc. New York

2. Stainer R.Y., Ingraham J.L., Wheelis M.L. and Painter P.R.[1999] GENERAL MICROBIOLOGY-Macmillan Education Ltd. London

3. Roitt I.M. [2000] Essential Immunology. Blackwell Scientific Publishers

4. Kuby, J. [1999] Immunology W.H.Freeman and company, New york.

5. Higgins L.I, Best G.J and Jones J [1996] Biotechnology - principles and applications Blackwell Scientific publications Oxford London

6. Gupta P.K. Elements of Biotechnology [2001] Rastogi Publications, Meerut.

Institute of Microbial Technology (IMTECH), Chandigarh, Punjab
Admission To Ph.D. Program : Walk in Interview on July 28, 2008