UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN Zoology
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)

SYLLABUS

Semester VI - Practical III - Cell Biology, Genetics, Biotechnology, Microbiology and Animal Physiology

Duration of Examination: 3 hrs
Maximum Marks: 100
Credits: 4

A - CELL BIOLOGY

1. Micrometry - Use of Microscopes, Cameralucida, Stage and Ocular Micrometer.

2. Blood Smear preparation - Differential Count of WBC.

3. Counting of RBC and WBC using Haemocytometer (Demonstration only)

4. Mounting buccal epithelium and observing living cells using vital siaining.

5. Mitosis in Onion root tip squash

6. Meiosis in grasshopper testis squash

7. Study of prepared slides of histology

B - GENETICS

8. Study of the biology of Drosophila

9. Observation of Common mutants of Drosophila

10. Chironomous - Salivary gland Chromosome squash preparation

11. Human blood grouping

C - BIOTECHNOLOGY AND MICROBIOLOGY

12. Study of prepared slides, Models exhibits or specimen. (a) Escherichia coli (b)Bacteriophage (c)Plasmid (d) cDNA Library

13. Preparation of media and culture of microorganisms on Agar plates. (Demonstration only)

14. Demonstration of P.C.R. technique : Southern, Western and Northern blots

15. Visit to a Biotechnology lab and report.

D - ANIMAL PHYSIOLOGY

16. Use of Kymograph unit, B.P. apparatus, Respirometer.

17. Survey of Digestive enzymes in cockroach

18. Study of Ciliary activity in Fresh water mussel in relation to temperature.

19. Estimation of Oxygen consumption in a fish with reference to-body weight

20. Detection of nitrogenous waste products in fish tank water, frog tank water, bird excreta and mammalian urine

Human Salivary amylase activity in relation to pH