UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Semester IV - Paper IV - Biophysical and Biochemical Techniques II

Duration: 3 hrs
Maximum Marks: 100
Credits: 4

(90 hrs)

Unit I

Basic principles of electromagnetic radiation.
Energy, wavelength, wave numbers and frequency.
Absorption and emission spectra. Beer_ Lambert law, light absorption and its transmittance. UV and visible spectrophotometry - principles, instrumentation and applications on enzyme assay and kinetic assays, protein structural studies, nucleic acid structural studies. (20 hrs)

Unit II

Spectrofluorimetry - principles, instrumentation and applications in vitamin assays (riboflavin and thiamine), enzyme assays, fluoresent probes in the study of protein and membrane structure.

Flame photometry - principles, instrumentation and applications in trace elements (Sodium, potassium analysis).

Atomic absorption and spectrophotometry - principles, instrumentation and applications. (20 hrs)

Unit III

Atomic structure, radiation, types of radioactive decay, half life, units of radio activity. Detection and measurement of radioactivity - methods based upon ionization (GM counter), methods based upon excitation (Scintillation counter).

Autoradiography and isotope dilution techniques. Applications of radioisotopes in the elucidation of metabolic pathways, clinical scanning and radio dating.

Biological hazards of radiation and safety measures in handling radio isotopes. (20 hrs)

Unit IV

Computer fundamentals, introduction to Computer, Classification of digital computer systems, anatomy of digital computers, Computer architecture, Number system, Memory units, Auxiliary storage devices, Input devices, Output devices, Operating Systems, Programming languages, Introduction to data base management systems, Fox pro applications. (15 hrs)

Unit V

Windows 98 - features, Ms word , Word Basic, creating a document! template, mail merge, Tamil word processor.

Introduction to Excel - Data sort, data filters , valid criteria, invalid criteria, redundant crteira, functions.

Workng with Power Point - Parts of power point, windows - various toolbars, slide manipulation, fonts, graphs, tables and template.

Introduction to telecommunication - computer network, communication systems, distributor systems, internet and world wide web, electronic mail and intranets. (15 hrs)

BOOKS RECOMMENDED

1. A biochemical Guide to principles and techniques of practical biochemistry - Keith Wilson and Kenneth H. Goulding
2. Principles and techniques of practical biochemistry - Bryan L. Williams and. Keith Wilson.
3. Practical Biochemistry - Shawney
4. Physical Biochemistry - David Friefelder
5. Instrumental methods of Analysis - Chatwal - Anand
6. A first course in computer - Sanjey Saxena (unit II, unit III and unit IV)
7. Fundamentals of information technology (Unit I, Unit V) - Alexis Leon and Mathew Leon.
8. Kurinji Tamil software
9. Manuals of Biochemistry - Sathya Narayana