UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Semester V - Paper V - Enzymes and Intermediary Metabolism-I

Duration: 3 hrs
Maximum Marks: 100
Credits: 4

Unit I

ENZYMES

Introduction - chemical nature and general characterization - nomenclature, IUB system of enzyme classification, specificity, enzyme units, active site, mode of action - Lock and key theory and induced fit theory.

Enzyme Kinetics - Introduction to chemical kinetics, rate and order of reactions, factors affecting the enzyme activity, derivation of Michaelis - Menton Equation. Line - Weaver and Burk plot, Eadie- Hofstee plot. Enzyme inhibition - Competitive, non- competitive and uncompetitive inhibitions (kinetic derivations not required). (12 hrs)

Unit II

BIOLOGICAL OXIDATION

Introduction -free energy - free energy of hydrolysis of ATP and other organophosphates.

Role of High energy compounds - Electron transport chain- Components and reactions of ETC. Role of ETC - Oxidative Phosphosylation - Chemi Osmotic hypothesis. P/O ratio, uncouplers of oxidative phosphorylation. (12 hrs)

Unit III

Introduction to intermediary metabolism. The basic metabolism pathways,anabolic, catabolic and amphibo lic pathways.

Approaches to biochemical investigations, perfusion of isolated organ, slice techniques, trace techniques and mutant studies for elucidation of metabolic pathways. (12 hrs)

Unit IV

Carbohydrate metabolism - fate of absorbed carbohydrates. The glycolytic pathway - aerobic and anaerobic glycolysis, energitics, glucogenolysis, regulation of glygogen metabolism, citric acid cycle and its regulation. (12 hrs)

Unit V

Pentose phosphate pathway. Uronic acid pathway. Gloxylate cycle - Entner - Duodroff pathway. Fate of fructose, galactose and mannose. Photosynthesis-light and dark reactions. (12 hrs)

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