UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Semester V - Paper VII - Molecular Biology I

Duration: 3 hrs
Maximum Marks: 100
Credits: 4

Unit I

DNA as the vehicle of inheritance- Experimental evidence -Griffith, McLeod, McCarty and Avery,Herschey-Chase experiments.

Definition of Gene,organization of genes and non-coding DNA in prokaryotes and Eukaryotes - unique, moderately repetitive and highly repetitive DNA sequence, Satellite DNA.Cot value. (12 hrs)

Unit II

DNA replication in prokaryotes, mode of replication, Semiconservativo modes of replication. An overview of replication-replication eye, replication forks, semi discontinuous replication, Okazaki fragments, RNA primers-Enzymes of replication- DNA polymerases I, II, III, Topoisomerases, Helicases binding proteins and ligases. (12 hrs)

Unit III

θ Replication in E. coli-replisomes,event at OriC(initaion), events on the replication fork (elongation) and termination.Fidelity of replicaion σ (sigma) or rolling circle mode of replication in φ 4 x174.Eukaryotic DNA polymerases. Inhibitors of replication.(12 hrs)

Unit IV

Repair of DNA - types of damages,repair by direct reversal of damage, excision repair, recombination reopair, SOS repair. Mutation-definition, type of mutations such as (spontaneous and induced) point mutation.

Gene mutation and chromosomal aberrations. Cause of mutation-chemical and physical agents. (12 hrs)

Unit V

Recombinant DNA technology-Role of restriction endo-nucleases,plasmid and cosmid cloning vectors.

Brief outline of molecular cloning.Applications or recombinant DNA technology. (12 hrs)

BOOKS RECOMMENDED