UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Semester II - Practical I

Duration: 6 hrs
Maximum Marks: 100
Credits: 4

(90 hrs)

Practical

Note
1. Students should know the principles, theory and calculations of each experiments.
2. They should know to prepare all the solutions by themselves. They should standardize their solutions individually.

Practical I (90 hrs)

1. EXPERIMENT INVOLVING TITRIMETRIC PROCEDURES

a. Estimation of amino acids by formal titration.
b. Estimation of ascorbic acid by titrimetric method using 2,6 -dichiorophenol indophenol.
c. Determination of saponification value of an edible oil.
d. Determination of Acid number af an edible oil.
e. Estimation of reducing sugar from biological fluids by Benedict's titrimetric method.
f. Iodine value of an oil.

2. QUALITATIVE ANALYSIS.

a. Reactions of simple sugars including glucose, fructose, galactose. mannose, pentose, maltose, sucrose, lactose, starch, glycogen and dextrin.
b. Reactions of proteins - solubility, Biuret. Millon's xanthoprotcic test. denaturation by heat, pH change and precipitation by acidic reagents. Color reactions of amino acids like tryptophan, tyrosine, cysteine, methionine, arginine, proline and histidine.
c. Reactions of lipids - solubility, saponification tests for unsaturation, Liebermann Burchard test for cholesterol.