UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Application Oriented Subject II - Practical III

Duration: 6 hrs
Maximum Marks: 100
Credits: 4

(120 hrs)

1. COLORIMETRIC ESTIMATION

a. Estimation of creatinine by Jaffe' s method.
b. Estimation of urea by Diacetyl monoxime method.
c. Estimation of DNA.
d. Estimation of RNA.

2. CHROMATOGRAPHY SEPARATION

a. Paper chromatographic separation and detection of amino acids and simple sugars.
b. Chromatographic separation of chlorophyll, carotenes of flower pigments and protein using column.
c. Separation of polar and non-polar lipids by thin layer chromatography.

3. ELECTROPHORETIC TECHNIQUE

a. Separation of protein by SDS-PAGE.

4. EXPERIMENTS ON ENZYMES BY COLORIMETRY

a. Effects of pH, temperature and substrate concentration for amylase.
b. Assay of activity of serum Transaminases (SGOT-SGPT)

5 HAEMATOLOGY

RBC count, PCV, ESR, total and differential WBC count.