UNIVERSITY OF MADRAS
B.Sc. DEGREE COURSE IN BIO-CHEMISTRY
SEMESTER SYSTEM WITH CREDITS
(Effective from the Academic Year 2003-2004)
SYLLABUS

Application Oriented Subject II - Practical IV

Duration: 6 hrs
Maximum Marks: 100
Credits: 4

(120 hrs)

1. COLORIMETRIC ESTIMATION

a. Estimation of glucose by
i) Folin Wu
ii) O- Toluidine methods
b. Estimation of Albumin and A/G ratio in serum
c. Estimation of cholesterol by Zak's method

2. EXPERIMENTS ON ENZYMES BY COLORIMETRY:

a. Effects of pH, temperature and substrate concentration of Urease.
b. Assay of activity of alkaline phosphatase in serum.
c. Estimation of nitrogen by MicroKjeldahl distillation procedure.

3. URINE AND FAECES ANALYSIS:

a. Collection of urine and faecal samples.
b. Faecal analysis to detect fats, undigested food and blood.
d. Qualitative analysis of urine for normal and pathological conditions.

BOOKS RECOMMENDED:

1. Practical Clinical Biochemistry - Harold Varely.
2. Medical Laboratory Technology - Volume I, II, III - L.Mukerjee.
3. Clinical Chamistry- Ranjana Chawla.
4. Laboratory Manual in Biochemistry - Jayaraman.
5. Biochemical methods - S.Sadasivam and Manickam.
6. Introduction to practical biochemistry - David T. Plummer.