University of Madras - Test Papers of Bachelor of Science (BSc) Botany - Model Question Paper - Application Oriented Subject I - Paper IX - Micro Technique
Model Question Paper - Application Oriented Subject I - Paper IX - MICRO TECHNIQUE
Time 3 Hrs. Maximum 100 Marks
Draw Diagrams wherever necessary
Section - A (10 x 3 = 30 marks)
Answer any 10 question Each answer should not exceed 50 words
1. Smear
2. FAA
3. Squash technique
4. Mordant
5. Infiltration
6. Sledge microtome
7. Phase contrast microscopy
8. Dehydration
9. Uses of condenser
10. Vital stains
11. Carnoy's fluid
12. Micrometry
Section-B (5 x 6 = 3OMarks)
Answer any 5 questions
All questions carry equal marks Each answer should not exceed 200 words
13. State the advantages and limitations of electron microscopy
14. Describe the structure and function of a rotary microtome.
15. What do you mean by fixation? Give two examples
16. What is embedding? How is it carried out?
17. Explain the whole mount preparation of algae
18. Describe Feulgen's reaction and Feulgen stain
19. Explain the procedure to stain bacteria.
Section - C (4 x 10 = 40 Marks)
Answer any 4 Questions:
All questions carry equal marks each answer should not exceed 500 words:
20. Trace the pathway of light in a light microscope
21. Write in detail about preparation of section by praffin method
22. Give a detailed procedure for sectioning and staining of an angiosperm method by using microtome
23. Describe the procedure and chemicals used for maceration
24. Write the various types of ocular and objective lenses and add a note on its uses
25. Discuss the merits and demerits of various microtomes studied by you.